Laboratory Sciences Core (D) : Lab Services

Lab Services

Laboratory Core services are provided to assist investigators and fulfill CFAR objectives and scientific areas of research.

Following is a list of services, currently provided:

  • Human primary cell preparation of PBMC, T cells, B cell subsets and other cell types.
  • Evaluation of cytokines and soluble mediators in plasma, serum or supernatants by Luminex Magpix, cytometric bead array and ELISA.
  • Flow cytometry assays (4-12 color staining) including intracellular staining and T and B cell phenotype and function (in collaboration with the Sylvester Comprehensive Cancer Center Flow Cytometry Core).
  • ELISPOT assays for antigen specific T cell (IFN-γ, IL-2) nd B cell (antibody) secretion
  • TREC assays for T cell receptor excision circles
  • Dendritic cell and Natural Killer cell phenotype and function assays
  • Gut microbial translocation marker evaluation including LPS, 16s ribosomal DNA, and soluble CD14
  • Biorepository development for investigator-initiated studies. Repository management using LDMS system for liquid nitrogen/ultralow freezer storage of cells, plasma, PAXgene samples of RNA or DNA.
  • Multiplex realtime PcR for sincle cells or cell populations using the Fluidigm BioMarkHD and CI platforms.
  • Partnership with Dr. Mario Stevenson virology lab to provide elutriated human monocytes, macrophage/DC culture platforms, siRNA/shRNA services, Lentivirus vector production, viral cDNA intermediates, primary cell replication assays, Digital Droplet PcRand HIV-1 infectivity assays. The CFAR will offer a monocyte elutriation service, Monocytes and PBMCs with the charges at $20 per 1 million cells and PBLs at $10 per million cells, plus a $25 processing fee. Digital PCR service: Sample preparation (DNA extraction) $35 for UM users, $40 for non UM users, and ddPCR analysis $40 for UM users, $80 for non UM users. For more information please contact Jessica Salinas JSalinas@med.miami.edu
    We welcome consultation, especially prior to project development. Please contact us so that we can assist with planning and scheduling.